不同杀虫剂处理对赤拟谷盗P450基因诱导表达特性影响

害虫细胞色素P450基因可被杀虫剂迅速诱导,然而当前对不同杀虫剂处理下赤拟谷盗P450基因诱导表达特性的研究较少。本研究首先通过序列比对选取了来自不同家族的8个赤拟谷盗P450基因CYP4G7、CYP4Q4、CYP4BR3、CYP12H1、CYP6BK11、CYP9D4、CYP9Z5和CYP345A1,然后采用四种不同杀虫剂氯氰菊酯、氟氯氰菊酯、氯菊酯和吡虫啉对赤拟谷盗20 d幼虫进行生物测定,再根据生测结果以四个药剂亚致死剂量分别处理幼虫,并采用荧光定量PCR分析8个P450基因的表达特性。结果表明,CYP4G7和CYP345A1可以分别被氯氰菊酯(分别上调1.97倍和2.06倍)、氟氯氰菊酯(2.00倍和2.03倍)和氯菊酯(1.73倍和1.81倍)显著诱导,而CYP4BR3和CYP345A1可以被吡虫啉(分别上调1.99倍和1.93倍)显著诱导。本研究结果表明赤拟谷盗P450基因的显著诱导与基因家族类型以及农药品种有关。

Effects of Different Pesticides on Induction Expression Characteristic of Cytochrome P450 Genes in Tribolium castaneum

Pest cytochrome P450 gene can be induced rapidly by pesticides, however, few studies have been conducted on the induction expression characteristics of P450 gene in Tribolium castaneum under different pesticide treatments. In this study, we first selected eight P450 genes of Tribolium castaneum from different families by sequence alignment, which were CYP4 G7, CYP4 Q4, CYP4 BR3, CYP12 H1, CYP6 BK11, CYP9 D4, CYP9 Z5 and CYP345 A1, and then used four different insecticides cypermethrin, cyhalothrin, permethrin and imidacloprid to bioassay the 20 d larvae of Tribolium castaneum. Then, according to the results of bioassay, larvae were treated with sublethal doses of four insecticides and the expression characteristics of eight P450 genes were analyzed by real-time PCR. The results showed that CYP4 G7 and CYP345 A1 could be significantly induced by cypermethrin(up-regulated 1.97 and 2.06 times, respectively), cyhalothrin(2.00 and 2.03 times) and permethrin(1.73 and 1.81 times), whereas CYP4 BR3 and CYP345 A1 could be significantly induced by imidacloprid(up-regulated 1.99 and 1.83 times, respectively). Our results indicated that the significant induction of P450 gene in Tribolium castaneum might be related to gene family type and pesticide varieties.