Solubilization of the starch-granule-bound starch synthase of normal maize kernels |
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Authors: | Macdonald F D Preiss J |
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Institution: | Department of Biochemistry and Biophysics, University of California, Davis, California 95616 |
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Abstract: | The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significant activity with UDPglucose. |
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