细胞电融合法生产杂交瘤

细胞电融合过程包括使用交流电场使悬浮在缓冲液中的细胞排列成串,然后高压脉冲电流作用使细胞膜发生瞬时破裂,相邻细胞的细胞膜融合,继续受交流电场作用完成细胞融合。已经证明细胞电融合技术可用于杂交瘤生产,单克隆抗体生产以及细胞表面标记物细胞间的传递过程。

Formation of hybridomas by cell-cell electrofusion

The process of cell-cell electrofusion involves forcing cells into tight intercellular contact and then inducing fusion by delivering electric pulses to the cells. Cell-cell electrofusion has proven to be a useful and practical tool for the formation of hybridomas, the production of monoclonal antibodies, and the transfer of membrane surface markers from one cell to another.