Enzymic thin film coatings for bioactive materials |
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Authors: | Tong Xiaodong Trivedi Archana Jia Hongfei Zhang Minjuan Wang Ping |
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Institution: | Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108, USA. |
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Abstract: | Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 microg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film. |
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