Ribotyping of Burkholderia mallei isolates |
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Authors: | Harvey Steven P Minter Jennifer M |
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Affiliation: | US Army Edgewood Chemical Biological Center, 5138 Blackhawk Road, Aberdeen Proving Ground, MD 21010-5424, USA. steve.harvey@us.army.mil |
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Abstract: | In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world. DNA samples from each isolate were digested separately with PstI and EcoRI enzymes and probed with an Escherichia coli-derived 18-mer rDNA sequence to identify diagnostic fragments. Seventeen distinct ribotypes were identified from the combined data obtained with the two restriction enzymes. The results demonstrate the general utility of ribotyping for the subspecies identification of B. mallei isolates. |
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Keywords: | Burkholderia mallei Ribotyping Genotyping DNA polymorphisms Ribosomal Subspecies identification |
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