Localization of the proteinase-induced thiol groups in alpha 2-macroglobulin |
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Authors: | F Pochon V Favaudon J Bieth |
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Affiliation: | 1. U. 219 INSERM, Institut Curie - Biologie, Laboratoires 110–112, Centre Universitaire, 91405 Orsay, France;2. U. 237 INSERM, Faculté de Pharmacie, Université Louis Pasteur, 67048 Strasbourg, France |
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Abstract: | Free thiol groups released on proteolytic attack of alpha 2-macroglobulin by trypsin or chymotrypsin bind covalently to thiopropyl-Sepharose, indicating that they are located at the surface of the complexes. These cysteine sulfhydryl groups appear to be in contact with the alpha 2M-bound proteases from singlet-singlet energy transfer measurements between fluorescein isothiocyanate-labeled proteinases and N-(iodoacetylaminoethyl)-5-naphtylamine-1-sulfonic acid-labeled thiols in alpha 2-macroglobulin. |
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