一个新的人类N末端乙酰转移酶基因的克隆及其表达

乙酰化修饰普遍发生于真核生物的各种蛋白质 ,对蛋白质的稳定性和活性产生重大影响。酵母N末端乙酰基转移酶NatA由NAT1和ARD1两个亚基组成。任何一个亚基缺陷都导致NatA活性丧失 ,酵母表现出在接合型转换、细胞周期调控及染色体稳定性等方面的异常。通过同源克隆方法 ,克隆了与酵母NAT1高度同源的一个人类基因。经GenBank查询 ,为新基因 ,命名为HNAT1。原位杂交结果显示 ,在成年小鼠曲精管内 ,该基因的表达强烈。其表达强度与细胞类型和细胞所处时相有关。该基因在精子发生过程中可能具有重要作用

Cloning and Analysis of a Novel Gene Encoding N-terminal Acetyltransferase Subunit

N-terminal acetylation is the most common modification in eukaryotic proteins, affecting stability and activity of proteins. NatA is one of the N-terminal acetytransferases in yeast. It is composed of two subunits, NAT1 and ARD1. Defect in one of them leads to loss of activity of NatA. Null mutant of NAT1 in yeast exhibits a variety of phenotypes, including depression of a silent mating type locus (HML), failing to enter G 0 in poor nutrient situations and chromosomes instability. Based on homology of NAT1 between yeast and other organisms, the full-length CDS (coding sequence) of HNAT1 was cloned and sequenced. Result of in situ hybridization in testis of rat showed that expression of NAT1 was high and its expression was different in different phases of spermatogenesis. The gene may play an important role in spermatogenesis.