新疆分离的0507JS60鉴定为辽宁病毒

0507JS60是从中国新疆喀什地区采集的库蚊标本分离的病毒株,可以在C6/36细胞上稳定传代.电镜观察显示完整病毒颗粒呈球形,直径55nm(n=10),无包膜,衣壳表面壳粒结构非常清晰.基因组核酸电泳显示基因组包括12条双链RNA(Double stranded RNA,dsRNA)片段.病毒第12基因片段序列测定结果显示该片段全长760bp(GenBank ID:FJ157354),具有长度为525bp的单一开放读码框(Single open reading frame,ORF),编码长度为174个氨基酸的蛋白,分子量约18.9kD.病毒第12基因片段序列比对显示与辽宁病毒(Liaoning virus,LNV)核酸序列同源性大于89%,氨基酸序列同源性大于91%.病毒第12基因片段系统进化分析显示该病毒与LNV病毒位于同一进化分枝内,与LNV-NE9712病毒株进化关系更接近.0507JS60经系统鉴定为LNV,这是首次在东北以外的地区分离到该病毒.

0507JS60 Virus Isolated in Xinjiang was Identified as Liaoning Virus

0507JS60 virus was isolated from a pool of Culex sp.collected in Kashi,Xinjiang,which could be propagated stably on C6/36 cells and caused cytopathic effects continuously.Viral particles had no envelope and appeared round with diameter of about 55nm(n=10).Capsomeres on the surface of capsid were clearly visible.Electrophoresis of viral genome showed a profile of 12 double stranded RNA(dsRNA) segments.Sequencing of the twelfth segment revealed the length of 760bp(GenBank ID:FJ157354).A single open reading frame(ORF) was found and encoded a protein of 174 amino acids with a molecular mass of 18.9kD.The nucleotide sequence had similarity over 89% with that of LNV,but the deduced amino acid sequence had similarity over 91% with that of LNV.A phylogenetic tree was constructed to compare the corresponding genetic sequences in Seadornavirus.The tree demonstrated that 0507JS60 virus lied in the same branch with LNV and more closely related to LNV-NE9712.0507JS60 virus was identified as LNV,which was firstly isolated outside the Northeast of China.