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Quantitative production of 2-acetamido-2-deoxy-D-glucose from crystalline chitin by bacterial chitinase |
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| Authors: | Pichyangkura Rath Kudan Sanya Kuttiyawong Kamontip Sukwattanasinitt Mongkol Aiba Sei-ichi |
| Affiliation: | Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. rath.p@chula.ac.th |
| Abstract: | Finely powdered alpha- and beta-chitin can be completely hydrolyzed with chitinase (EC 3.2.1.14) and beta-N-acetylhexosaminidase (EC 3.2.1.52) for the production of 2-acetamido-2-deoxy-D-glucose (GlcNAc). Crude chitinase from Burkholderia cepacia TU09 and Bacillus licheniformis SK-1 were used to digest alpha- and beta-chitin powder. Chitinase from B. cepacia TU09 produced GlcNAc in greater than 85% yield from beta- and alpha-chitin within 1 and 7 days, respectively. B. licheniformis SK-1 chitinase completely hydrolyzed beta-chitin within 6 days, giving a final GlcNAc yield of 75%, along with 20% of chitobiose. However, only a 41% yield of GlcNAc was achieved from digesting alpha-chitin with B. licheniformis SK-1 chitinase. |
| Keywords: | Chitin Chitinase N-Acetyl- smallcaps" >d-glucosamine 2-Acetamido-2-deoxy- smallcaps" >d-glucose Bacillus licheniformis Burkholderia cepacia |
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