无载体框架序列转基因小麦中外源1Ax1基因表达框的遗传分析

为了研究无载体框架序列转基因小麦中转基因表达框的遗传规律，选育稳定表达的转基因株系，利用基因枪介导1Ax1基因的最小表达框转化得到了转基因小麦，对其后代转基因植株中1Ax1基因表达框的遗传进行了分析，结果表明：无载体框架结构的1Ax1基因在转基因后代中稳定遗传，在T1代中呈现3:1的分离，遵从孟德尔遗传模式；SDS－PAGE分析表明其中部分转基因后代分离出新的高分子量蛋白亚基分子量略低于1Dx5亚基。

Inheritance of transgene 1Ax1 in transgenic wheat (Triticum aestivum L.) with linear transgene lacking vector backbone sequence

Transformation with gene minimal expression cassettes without vector backbone sequence is a new transformation technique. In the current research, the inheritance of the foreign 1Ax1 gene cassettes were analyzed in transgenic plants recovered with gene cassettes lacking vector backbone sequences. The results showed that linear 1Ax1 gene cassettes were stably expressed and separated at the rate of 3 to 1 in the seeds of T, generation. It suggested the functional copies of 1Ax1 gene cassettes were integrated in one sites, abiding by Mendelian genetic law. It revealed firstly the feasibility of the transformation technique with gene cassettes, and proved the inheritance of transgene cassettes in subsequent generations.