Studies on the sucrose phosphate synthetase kinetic mechanism |
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Authors: | Graciela L Salerno Horacio G Pontis |
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Institution: | Departamento de Biología, Fundación Bariloche, C.C. 138, 8400 San Carlos de Bariloche, Argentina |
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Abstract: | The kinetic properties of wheat germ sucrose phosphate synthetase, which catalyzes the reaction UDP-glucose + fructose 6-phosphate → UDP + sucrose 6-phosphate have been studied. A plot of the reciprocal initial velocity versus reciprocal substrate concentration gave a series of intersecting lines indicating a sequential mechanism. Product inhibition studies showed that UDP was competitive with UDP-glucose and noncompetitive with fructose 6-phosphate. A dead-end inhibitor, inorganic phosphate, was competitive with UDP-glucose and noncompetitive with fructose 6-phosphate. The results of initial velocity and product and dead-end inhibition studies suggested that the addition of substrates to the enzyme follows an ordered mechanism. |
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