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Improved Human Sperm Recovery Using Superoxide Dismutase and Catalase Supplementation in Semen Cryopreservation Procedure
Authors:Tiziana Rossi  Fernando Mazzilli  Michele Delfino  Franco Dondero
Institution:Department of Medical Pathophysiology, Laboratory of Seminology and Immunology of Reproduction, University 'La Sapienza', Policlinico Umberto I, 00161 Rome, Italy.
Abstract:The aim of this work was to evaluate the effects of ROS scavenger supplementation in human semen samples undergoing cryopreservation procedures.After screening out andrological pathologies, we selected 25 male partners of infertile couples with the following semen profile: volume >/= 2.0 ml, normal viscosity, sperm count >/=20 x 10(6)/ml, straight progressive motility (classes 1 and 2) >/= 40% (Mazzilli, Rossi, Delfino and Nofroni (1999) Andrologia 31: 187-194), atypical forms
Keywords:catalase  cryopreservation  reactive oxygen species (ROS)  semen  superoxide dismutase (SOD)  superimposed image analysis system
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