恶性疟原虫杂合多肽抗原融合基因的克隆和表达

合成了５对寡核苷酸片段，分别连接在两种恶性疟原虫杂合多肽（４５肽和５８肽）抗原基因片段（ＨＰＦＧＡ和ＨＰＦＧＢ）的头部和尾部，将这两种片段分别与霍乱毒素Ｂ亚单位（ＣＴ－Ｂ）基因末端融合。两种杂合多肽抗原分别含有数个红内期和红外期有代表性的、并能被Ｔ或Ｂ淋巴细胞识别的保护性抗原表位，ＣＴ－Ｂ基因前端具有促使分泌的信号肽序列。将这两种融合基因的不同重组质粒分别转化大肠杆菌，对转化菌的培养上清检测表明融合蛋白被分泌性表达，既具有恶性疟原虫和ＣＴ－Ｂ抗原性，又保持了ＣＴ－Ｂ与其受体神经节苷脂ＣＭ１结合的生物学活性。

Cloning and Expression of Complex Antigen Fusion Genes of Plasmodium falciparum

Five pairs of chemically synthesized complementary oligonucleotides were linked differently to both ends of two chemically synthesized gene fragments of comlex antigens which contained representative protective epitopes of exo-erythrocytic and erythrocytic stages of Plasmodium falciparum. Then the two reconstructed complex antigen gene fragments were respectively cloned and fused in correct open reading frame with cholera toxin B subunit(CT-B) gene preceded by a signal peptide sequence. After the new recombinant plasmids were transformed into E.coli,the fusion proteins could be specifically expressed.secreted to the medium and also preserved the biological activity of CT-B that binding with its receptor GM1.