Rapid clonal propagation of three mulberries,Morus cathayana HemsL,M. lhou Koiz. andM. serrata Roxb., through in vitro culture of apical shoot buds and nodal explants from mature trees |
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Authors: | S K Pattnaik P K Chand |
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Institution: | (1) Plant Tissue and Cell Culture Facility, Post-Graduate Department of Botany, Utkal University, Bhubaneswar-751 004, Orissa, India |
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Abstract: | High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5–1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical shoot buds and nodal explants and also enhanced the frequency of bud break in all three species. Shoot culture initiation was greatly influenced by explant type, explant age and explanting season. The shoots were successfully rooted on half-strength MS medium containing a combination of indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid, each at 1.0 mg/l. The plantlets were successfully acclimated and eventually established in soil.Abbreviations
BAP
6-Benzylaminopurine
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GA
3
Gibberellic acid
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IAA
Indole-3-acetic acid
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IBA
Indole-3-butyric acid
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IPA
Indole-3-propionic acid
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Kn
Kinetin
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MS
Murashige and Skoog (1962) medium
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NAA
1-Naphthalene acetic acid |
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Keywords: | In vitro propagation M cathayana M lhou M serrata Mulberries |
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