Detection of Aspergillus in lung and other tissue samples using the MycAssay Aspergillus real-time PCR kit |
| |
Authors: | Lass-Flörl C Follett S A Moody A Denning D W |
| |
Institution: | Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Fritz-Pregl-Stra?e 3, 6020 Innsbruck, Austria. cornelia.lass-floerl@i-med.ac.at |
| |
Abstract: | The MycAssay? Aspergillus real-time PCR kit was tested on tissues from patients with invasive fungal infections. Tissue samples from nine organ transplant recipients and 33 patients with haematological malignancy were from lung (n = 30), skin (n = 4), and others. Samples were preprocessed with proteinase K and lyticase, followed by DNA extraction and real-time PCR. For all samples, the sensitivity of the MycAssay Aspergillus test was 82% and specificity 79% relative to microscopy and 90% and 64%, respectively, compared with Aspergillus culture. The positive predictive value and negative predictive values compared with culture were 69% and 88% and were 88% and 69% compared with microscopy, respectively. The MycAssay Aspergillus test detected tissue invasive infections with Aspergillus fumigatus , Aspergillus flavus , and Aspergillus terreus. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|