Mutagenic Analysis of the F
0
Stator Subunits |
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Authors: | Brian D Cain |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, Gainesville, Florida, 32610 |
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Abstract: | The a and b subunits constitute the stator elements in the F0 sector of F1F0-ATP synthase.Both subunits have been difficult to study by physical means, so most of the information onstructure and function relationships in the a and b subunits has been obtained using mutagenesisin combination with biochemical methods. These approaches were used to demonstrate thatthe a subunit in association with the ring of c subunits houses the proton channel throughF1F0-ATP synthase. The map of the amino acids contributing to the proton channel is probablycomplete. The two b subunits dimerize, forming an extended flexible unit in the peripheralstalk linking the F1 and F0 sectors. The unique characteristics of specific amino acid substitutionsaffecting the a and b subunits suggested differential effects on rotation during F1F0-ATPaseactivity. |
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Keywords: | F1F0 ATP synthasem stator F0 |
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