Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L. |
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| Authors: | Nick J. Gawel Arelli P. Rao Carol D. Robacker |
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| Affiliation: | (1) Department of Plant and Soil Science, Texas Tech University, 79409 Lubbock, TX, USA;(2) Department of Horticulture, University of Georgia, 30212 Experiment, GA, USA |
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| Abstract: | Leaf discs from four strains and petioles from six strains of Gossypium hirsutum were cultured on a variety of media. Callus formed from explants on all media, though embryogenesis was highly specific. Embryos formed from only three strain x media combinations. A small percentage of these embryos developed into plantlets. These results demonstrate that cotton plants can be obtained from leaf tissue explants.Abbreviations BA benzyladenine - 2,4-D 2,4-dichlorophenoxyacetic acid - GA3 gibberellic acid - Kn Kinetin - NAA 1-naphthalene acetic acidCollege of Agricultural Sciences Publication Number T-4-193; this research was partially funded by the USDA-ARS Plant Stress and Water Conservation Research Program |
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