Factors influencing T-DNA transferring into pollen of lily in vitro |
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Authors: | W Dong Y F Mao W Li |
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Institution: | (1) Marine Biological Engineering Dept., Ocean University of China, Qingdao, 266003, China;(2) Institute of Cell Biology, Lanzhou University, Lanzhou, 730000, China |
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Abstract: | Direct pollen transformation method improves the classical transformation procedures because some tissue culture steps and
subsequent regeneration can be avoided. A critical step in the development of Agrobacterium-mediated transformation is the establishment of optimum conditions for T-DNA delivery into tissue. The pollen grains of David
lily (Lilium davidii Duchartre) are transformable by Agrobacterium during their germination, and extremely high GUS expression frequency of pollen had been achieved (92.7 ± 2.7%), but not
for the ungerminated pollen. The culture medium, Agrobacterium cell density, duration of co-cultivation, and the combination of bacterial strains and plasmids should be optimized to get
the highest transformation frequency. Thus, a method for pollen monocotyledonous species reproductive tissues transformation
by Agrobacterium in monocots has been successfully developed.
Published in Russian in Fiziologiya Rastenii, 2007, Vol. 54, No. 3, pp. 475–480
The text was submitted by the authors in English. |
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Keywords: | Lilium davidii Agrobacterium tumefaciens GUS transformation pollen |
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