Acetaminophen inhibits cytochrome c redox cycling induced lipid peroxidation |
| |
Authors: | Yin Huiyong Vergeade Aurélia Shi Qiong Zackert William E Gruenberg Katherine C Bokiej Magdalena Amin Taneem Ying Weizhen Masterson Tina S Zinkel Sandra S Oates John A Boutaud Olivier Roberts L Jackson |
| |
Institution: | Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA. diaswb@biof.ufrj.br |
| |
Abstract: | Recent evidence indicates that site-specific crosstalk between O-GlcNAcylation and phosphorylation and the O-GlcNAcylation of kinases play an important role in regulating cell signaling. However, relatively few kinases have been analyzed for O-GlcNAcylation. Here, we identify additional kinases that are substrates for O-GlcNAcylation using an in vitro OGT assay on a functional kinase array. Forty-two kinases were O-GlcNAcylated in vitro, representing 39% of the kinases on the array. In addition, we confirmed the in vivo O-GlcNAcylation of three identified kinases. Our results suggest that O-GlcNAcylation may directly regulate a substantial number of kinases and illustrates the increasingly complex relationship between O-GlcNAcylation and phosphorylation in cellular signaling. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|