The control of lysine biosynthesis in maize |
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| Authors: | RM Cheshire BJ Miflin |
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| Institution: | Department of Plant Science, University of Newcastle, Newcastle upon Tyne, and Biochemistry Department, Rothamsted Experimental Station, Harpenden, Herts, England |
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| Abstract: | Aspartate kinase has been partially purified and characterised from germinating maize seedlings. The Km for aspartate was 9 mM. Out of several amino acids which are potential feedback regulators of the enzymes, only lysine is markedly inhibitory, having a Ki of 13 μM and causing 100% inhibition at 0.5 mM. Lysine also protects the enzyme against heat inactivation. Dihydrodipicolinic acid synthase isolated from the same tissue is also inhibited by lysine, 1 mM causing 95% inhibition. |
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| Keywords: | Gramineae maize enzymes aspartate kinase dihydrodipicolinic acid synthase lysine biosynthesis control |
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