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Gas chromatography profile of estrogens: Application to pregnancy urine |
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| Authors: | GL Adessi D Eichenberger Tran Quang Nhuan MF Jayle |
| Institution: | UER Biomedicale des Sts peresFrance |
| Abstract: | A method for the simultaneous quantitation of 7 estrogens in pregnancy urine is described. It involves enzymatic hydrolysis, extraction of free steroids, ion-exchange column chromatography and quantitation of the trimethylsilyl derivatives by gas chromatography on OV 1. Data obtained from normal and twin pregnancies and from women with anencephalic fetus or intra uterine fetal death are analysed. The sensibility of the method is about 40 μg of each estrogen by liter of urine. |
| Keywords: | trivial and systematic names E1 estrone 3-Hydroxy-1 3 5 (10)-estratrien-17-one E 2 estradiol 17β 1 3 5 (10)-estratrien-3 17β -diol 2 met E 1 2-methoxy estrone 3-Hydroxy 2-methoxy-1 3 5 (10)-estra-trien-17-one 16 HO E 1 16 α-hydroxyestrone 3 16 α-Dihydroxy-1 3 5 (10)-estratrien-17-one 16 oxo E 2 16-oxo-estradiol 3 17 β -Dihydroxy-1 3 5 (10)-estratrien-16-one 16 17-epi E 3 16 17-epiestriol 1 3 5 (10)-estratrien-3 16 β 17 α-triol 17 epi E 3 17 epiestriol 1 3 5 (10)-estratrien-3 16 α 17 α-triol E 3 estriol 1 3 5 (10)-estratrien-3 16 α 17β-triol 16 epi E 3 16-epiestriol 1 3 5 (10)-estratrien-3 16 β -triol E 4 estetrol 1 3 5 (10)-es-tratrien-3 15 α 16 α 17 β -tetrol Epi epicoprostanol 5β -cholestane-3α o1 |
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