Abstract: | The administration of l-tryptophan-[3-14C] to Lupinus hartwegii (3-day-old seedlings and 8-week-old plants) resulted in the formation of gramine-[methylene-14C], indicating that gramine is produced by the same biosynthetic route in this species as in barley. Radioactive indole-3-aldehyde, labelled specifically on its aldehyde carbon, was isolated from the 8-week-old plants. However no significant amount of this compound was detected in 7-day-old seedlings, and it is suggested that indole-3-aldehyde is formed by the metabolism of gramine in the maturing plant. |