Mutation and nuclear stage in Schizosaccharomyces pombe I. An experimental approach to the role of recombination in mutation induction

A reverse mutation system using G1 and G2 cells of Schizosaccharomyces pombe is described. In order to enable the system to deal with the problem of mutation dependence on recombination, tests were performed on (i) the homogeneity of cell populations with respect to nuclear stage; (ii) the fate of cells during post-irradiation incubation; (iii) the colony-forming ability of G1 and G2 revertants, and (iv) cell viability on the mutation plates. On the basis of the results, it is thought that, using this system, information can be obtained on the role of recombinational events in the process of mutation induction.