Isoperoxidases from tobacco tissue cultures |
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Authors: | Bernard L Powell Jerry W Pickering Simon H Wender Eddie C Smith |
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Institution: | Department of Chemistry, Biochemistry Division, University of Oklahoma, Norman, Oklahoma 73069, U.S.A. |
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Abstract: | Two anodic isoperoxidases (A1 and A2) from tobacco tissue culture W-38 and two cathodic isoperoxidases (C3 and C4) from tobacco suspension culture WR-132 have been separated and characterized. Molecular weights for each of the isoperoxidases have been determined by two different methods. Only C4 contained a carbohydrate component. The substrate specificity and the pH optima for the four enzymes with each of five substrates were determined. |
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Keywords: | Salanaceae tobacco tissue culture isoperoxidases ferulic acid oxidation |
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