Free sterols,steryl esters,glucosides, acylated glucosides and water-soluble complexes in Calendula officinalis |
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Authors: | Gra?yna Adler Zofia Kasprzyk |
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Institution: | Department of Biochemistry, University 02-089 Warszawa, al. ?wirki i Wigury 93, Poland |
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Abstract: | In 3- and 14-day-old seedlings and in the leaves of Calendula officinalis the following sterols were identified: cholestanol, campestanol, stigmastanol, cholest-7-en-3-β-ol, 24-methylcholest-7-en-3β-ol, stigmast-7-en-3β-ol, cholesterol, campesterol, sitosterol, 24-methylcholesta-5,22-dien-3β-ol, 24-methylenecholesterol, stigmasterol and clerosterol. Sitosterol was predominant in young and stigmasterol in old tissues. Young tissues contained relatively more campesterol but in old tissues a C28Δ5,22 diene was present suggesting transformation of campesterol to its Δ5,22 analog, similar to that of sitosterol to stigmasterol. All the identified sterols were present as free compounds and also in the steryl esters, glucosides, acylated glucosides and water-soluble complexes. The variations in the amounts of these fractions in the embryo axes and cotyledons of 3- and 14-day-old seedlings and the distribution of individual sterols among the fractions are discussed. |
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Keywords: | Compositae identification of phytosterols steryl esters steryl glucosides acylated steryl glucosides water-soluble steryl complexes |
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