Cycloheximide resistance in Chinese hamster cells I. Spontaneous mutagenesis |
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| Authors: | Hubert Pöche Nina B. Varshaver Erhard Geissler |
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| Affiliation: | 1. Academy of Science of the GDR, Central Institute of Molecular Biology, Cell Genetics Department, Berlin-Buch, G.D.R.;1. Kurchatov Institute of Atomic Energy, Biological Department, Moscow U.S.S.R. |
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| Abstract: | Resistance to cycloheximide (CHM) was studied in cultured Chinese hamster cells. Concentrations of CHM above 5.10−7M were toxic for the cells. At concentrations above 9.10−7M no colonies were recovered in selective medium. 15 resistant clones of independent origin were isolated in selective medium containing 7.10−7M CHM. Resistance was stable when the cells were cultured under non-selective conditions. The spontaneously mutation rate was determined by the fluctuation test. Mutations to CHM resistance arose spontaneously. The spontaneous mutation rate to CHM resistance was about 10−5. |
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| Keywords: | CHM cycloheximide MEM minimal essential medium |
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