首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Proteomic analysis of the pathophysiological process involved in the antisnake venom effect of Mucuna pruriens extract
Authors:Guerranti Roberto  Ogueli Ifeanyi G  Bertocci Erica  Muzzi Chiara  Aguiyi John C  Cianti Riccardo  Armini Alessandro  Bini Luca  Leoncini Roberto  Marinello Enrico  Pagani Roberto
Institution:Department of Internal Medicine, Endocrine-Metabolic Sciences and Biochemistry, University of Siena, Via A. Moro 2, Siena, Italy. guerranti@unisi.it
Abstract:Previously, we reported the antisnake venom properties of a Mucuna pruriens seed extract (MPE) and tested its in vivo efficacy against Echis carinatus venom (EV) in short- (1 injection) and long-term (three weekly injections) treatments. The aim of the present study was to investigate plasma proteome changes associated with MPE treatments and identify proteins responsible for survival of envenomated mice (CHALLENGED mice). Six treatment groups were studied. Three control groups: one saline, one short-term and one long-term MPE treatment. One group received EV alone. Two test groups received EV with either a short-term or long-term MPE treatment (CHALLENGED mice). The plasma from each group was analysed by 2-DE/MALDI-TOF MS. The most significant changes with treatment were: albumin, haptoglobin, fibrinogen, serum amyloid A and serum amyloid P. Most of these changes were explained by EV effects on coagulation, inflammation and haemolysis. However, MPE treatments prevented the EV-induced elevation in HPT. Consequently, HPT levels were similar to controls in the plasma of CHALLENGED mice. The plasma of CHALLENGED mice showed substantial proteomic modifications. This suggests the mechanism of MPE protection involves the activation of counterbalancing processes to compensate for the imbalances caused by EV.
Keywords:2‐DE  Mouse plasma proteome  Mucuna pruriens  Plant extract  Snake venom
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号