Comparative study of the free amino acid compositions and contents in three different botanical origins of Coptis herb |
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| Affiliation: | 1. College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China;2. National Institute of Measurement and Testing Technology, Chengdu, 610021, China;1. School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo ku, Kumamoto, 862-0973, Japan;2. Program for Leading Graduate Schools, Health Life Science: Interdisciplinary and Glocal Oriented (HIGO) Program, Kumamoto University, Kumamoto, Japan;1. Key Laboratory of Marine Biogenetic Resources, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen, 361005, China;2. Yanjing Medical College, Capital Medical University, 4 Dadong Road, Beijing, 101300, China;1. Department of Plant Protection, University of Life Sciences in Lublin, Leszczyńskiego 7, 20-069, Lublin, Poland;2. Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110, Siedlce, Poland;3. Department of Molecular Biotechnology, Faculty of Biotechnology, University of Rzeszów, Pigonia 1, 35-310, Rzeszów, Poland;1. Dipartimento di Biologia Ambientale, Piazzale Aldo Moro, 5, 00185, Rome, Italy;2. Dipartimento di Chimica: Università di Roma “La Sapienza”, Piazzale Aldo Moro, 5, 00185, Rome, Italy;3. Dipartimento di Scienze Farmaceutiche: Università degli Studi di Milano, Via Mangiagalli 25, I-20133, Milano, Italy;4. School of Pharmacy: University of Camerino, Via S. Agostino, 1 - 62032, Camerino, Italy;1. Blagoveshchensk State Pedagogical University, Blagoveshchensk, Russian Federation;2. Education Scientific Center of Nanotechnology, Far Eastern Federal University, Vladivostok, 690950, Russian Federation;1. Department of Chemistry, Higher Teacher Training College, University of Yaounde I, P. O. Box 47, Yaounde, Cameroon;2. Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;3. H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan;4. Pharmaceutical Research Center, PCSIR Laboratories Karachi Complex, Karachi, 75280, Pakistan;5. Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon |
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| Abstract: | Free amino acids are important bioactive ingredients in herbs and herbal products. Coptis herbs contain a variety of free amino acids; however, studies have not yet analyzed the relationship between free amino acids and species of Coptis herbs. In the current study, the contents of 20 free amino acids in Coptis chinensis Franch., Coptis teeta Wall., and Coptis deltoidea C.Y. Cheng et Hsiao were determined using an automatic amino acid analyzer to evaluate the differences between the three species. We found that the major amino acids (Asn, Arg, and γ-aminobutyric acid (GABA)) were significantly more in C. chinensis than in the other two species. In addition, Asp content was significantly more in C. deltoidea. The study concluded that Asn can be used to identify C. deltoidea, C. chinensis, and C. teeta, and Gln and Arg can be used to distinguish C. teeta from C. chinensis and C. deltoidea. These findings suggested that free amino acids as active ingredients can be used to identify Coptis herb species. |
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| Keywords: | Free amino acids Automatic analyzer Species identification |
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