Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes |
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| Authors: | J Mukherjee S Majumdar T Scheper |
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| Institution: | Institut für Technische Chemie der Universit?t Hannover, Callinstr.3, D-30167 Hannover, Germany e-mail: mukherjee@mbox.iftc.uni-hannover.de Fax: +49-511-7623004 Tel.: +49-511-7622959, DE
Department of Life Science and Biotechnology, Jadavpur University, Calcutta 700 032, India, IN
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| Abstract: | The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate
(0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen catabolite repression of enzyme formation
was absent in this bacterium. Cultivation in a reactor showed that the dissolved oxygen level is the limiting factor for L-asparaginase
production by E. aerogenes. Glucose was found to be a repressor of enzyme synthesis. Asparagine was absent intracellularly when the L-asparaginase level
was high. An increase in the extracellular alanine level when the dissolved oxygen remained low indicated a shift from aerobic
to fermentative metabolism.
Received: 20 July 1999 / Accepted: 2 October 1999 |
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