Demonstration of G . U wobble base pairs by Raman and IR spectroscopy |
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Authors: | Ackermann T Gramlich V Klump H Knäble T Schmid E D Seliger H Stulz J |
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Institution: | Inst. Physik. Chemie, Universität Freiburg, W. Germany |
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Abstract: | When guanine and uracil form hydrogen bonds in the pairing scheme first proposed by Crick one would expect that poly(A,G) will form an unperturbed double helix with poly U at room temperature in a dilute electrolyte solution (0.1 M NaCl). We have demonstrated by Raman- and IR-spectroscopy that the secondary structure of poly(A.G) · poly U is very similar to the structure of poly A · poly U; only the thermal stability of the double helix seems slightly lower than the stability of poly A · poly U, whereas the average helix length is unaffected by the dispersed G · U base pairs. From our input ratio of guanine and adenine we estimate that about every fourth base pair is a wobble pair. |
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