| CRISPR/Cas基因编辑技术研究进展及其在斑马鱼中的应用 |
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| 引用本文： | 欧阳娟,薛松磊,周琦琦,崔恒宓. CRISPR/Cas基因编辑技术研究进展及其在斑马鱼中的应用[J]. 生物工程学报, 2020, 36(1): 1-12 |
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| 作者姓名： | 欧阳娟 薛松磊 周琦琦 崔恒宓 |
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| 作者单位： | 扬州大学 表观遗传学与表观基因组学研究所，江苏 扬州 225009,扬州大学 表观遗传学与表观基因组学研究所，江苏 扬州 225009,扬州大学 表观遗传学与表观基因组学研究所，江苏 扬州 225009,扬州大学 表观遗传学与表观基因组学研究所，江苏 扬州 225009 |
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| 基金项目： | 国家自然科学基金 (No. 81773013) 资助。 |
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| 摘 要： | 规律成簇间隔的短回文序列(Clustered regularly interspaced short palindromic repeats,CRISPR)是细菌和古菌中的获得性免疫系统,利用该系统能定点进行基因编辑。最近,科学家发现了新的CRISPR-associated (Cas)蛋白,其中由Cas12a介导的基因编辑能显著降低脱靶率。文中对CRISPR/Cas系统的发现历史、组成和分类、工作原理进行概述,并总结了该系统的最新研究进展及在斑马鱼Danio rerio中的应用。
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| 关 键 词： | CRISPR/Cas 基因编辑 斑马鱼 |
| 收稿时间： | 2019-05-08 |
Research progress and applications of gene editing technology CRISPR/Cas in zebrafish |
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| Juan Ouyang,Songlei Xue,Qiqi Zhou,Hengmi Cui. Research progress and applications of gene editing technology CRISPR/Cas in zebrafish[J]. Chinese journal of biotechnology, 2020, 36(1): 1-12 |
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| Authors: | Juan Ouyang Songlei Xue Qiqi Zhou Hengmi Cui |
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| Affiliation: | Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China,Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China,Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China and Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China |
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| Abstract: | Clustered regularly interspaced short palindromic repeats(CRISPR) are acquired immune system in bacteria and archaea. This system is used in site-directed gene editing. Recently, scientists discovered new CRISPR-associated(Cas) proteins, in which Cas12 a-mediated gene editing can significantly reduce the off-target rate. In this article, we review CRISPR/Cas system’s discovery of history, composition, classification, and working principle. The latest research progress of the CRISPR/Cas system, and its application in zebrafish are introduced. |
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| Keywords: | CRISPR/Cas gene editing zebrafish |
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