Monoamine oxidase and transaminase screening: biotransformation of 2-methyl-6-alkylpiperidines by <Emphasis Type="Italic">Neopestalotiopsis</Emphasis> sp. CBMAI 2030 |
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| Authors: | Jonas?Henrique?Costa Bruna?Zucoloto?da?Costa Derlene?Attili?de?Angelis Email author" target="_blank">Anita?Jocelyne?MarsaioliEmail author |
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| Institution: | 1.Institute of Chemistry, State University of Campinas—UNICAMP,Campinas,Brazil;2.Division of Microbial Resources, Chemical, Biological and Agricultural Pluridisciplinary Research Center—CPQBA,State University of Campinas—UNICAMP,Campinas,Brazil |
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| Abstract: | High-throughput screening detected transaminases (TAs) and monoamine oxidases (MAOs) in fungi by applying a fluorogenic probe. Strains F026, F037, F041, F053, and F057 showed the highest enzymatic conversions (31, 60, 30, 40, and 32%, respectively) and where evaluated for their ability to transform piperidines. Strain F053 (Neopestalotiopsis sp. CBMAI 2030) revealed unusual enzymatic activity to deracemize 2-methyl-6-alkylpiperidines. Neopestalotiopsis sp. CBMAI 2030 was capable to convert 2-methyl-6-propylpiperidine, 2-methyl-6-butylpiperidine, and 2-methyl-6-pentylpiperidine in piperideine with 11, 14, and 24% conversion, respectively. The activity was enhanced by cultivating the fungus with 2-methyl-6-pentylpiperidine (38% conversion and 73% ee). |
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