Improved method for the isolation of RNA from plant tissues |
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| Authors: | J Logemann J Schell L Willmitzer |
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| Affiliation: | 1. Laboratory of Hematology, University Hospital Saint-Louis, AP-HP, Paris, France;2. EA3518, University Paris Diderot, Paris, France;3. Imagene, Evry, France;4. Laboratory of Hematology, CHRU, Lille, France;5. Laboratory of Hematology, University Hospital, Bordeaux, France;6. Laboratory of Hematology, University Hospital, Toulouse, France;7. Laboratory of Hematology, University Hospital Necker-Enfants-Malades, AP-HP, Paris, France;8. Laboratory of Hematology, University Hospital, Lyon, France;9. Laboratoire de Biopathologie, Institut Paoli Calmettes, Marseille, France;10. Group of Molecular Biologists for Malignant Hemopathies, Toulouse, France;1. Department of Pharmacology, Physiology and Pathophysiology, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania;2. Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany;3. Department of Internal Medicine B, University Medicine Greifswald, Germany;4. Department of Toxicology, Faculty of Pharmacy, University of Medicine Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania;5. DZHK (German Center for Cardiovascular Research, Partner Site Greifswald), Germany |
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| Abstract: | A fast and efficient method for the isolation of RNA from plant tissues is described. Tuber tissue is homogenized in a guanidine hydrochloride-containing buffer followed by direct extraction with phenol/chloroform. The RNA is precipitated from the aqueous phase, washed with 3 M sodium acetate and 70% ethanol, and finally dissolved in water. The yield of RNA is up to 500 micrograms/g of tissue and several tests indicate intact and nondegraded RNA. This method can be adapted to a small-scale version by the use of 1.5-ml tubes, allowing rapid isolation of RNA from a larger number of samples. Finally, this method is of particular use for isolating RNA from tissues with a high polysaccharide and nuclease content such as wounded potato tubers. |
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