Developmental accumulation of hydroxyproline and hydroxyproline-containing proteins in Zea mays pollen |
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Authors: | Amy L Rubinstein Rogério T N Prata Patricia A Bedinger |
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Institution: | (1) Biology Department, University of North Carolina, 010A Coker Hall, CB # 3280, 27599-3280 Chapel Hill, NC, USA |
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Abstract: | The hydroxyproline content of developing Zea mays (maize) pollen was determined. The level of hydroxyproline in uninucleate microspores early in pollen development was low (0.004% of dry weight). In contrast, mature pollen is enriched for this amino acid (0.1% of dry weight). In mature pollen, 90% of the hydroxyproline is in the soluble fraction. Upon in vitro pollen germination, hydroxyproline associated with the insoluble fraction increased from 10% to 26% of the total hydroxyproline. Antibodies specific to extensins and arabinogalactan proteins (AGPs), two major classes of hydroxyproline-containing proteins, recognized two distinct groups of proteins in maize pollen by Western analysis. The two types of pollen hydroxyproline-containing proteins could also be distinguished based on their behavior upon anion exchange chromatography. |
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Keywords: | Hydroxyproline Pollen Maize Arabinogalactan protein Extensin |
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