天然吸附剂对大肠杆菌破碎液中蛋白质的吸附性能研究

采用硅藻土、活性白土、膨润土系列作吸附剂,对经过诱导的E.coliBL21(pQHP)发酵液经离心、破碎,制备成的蛋白备用液吸附后,离心分离出的上清液用Bradford法测定蛋白质浓度,以此来计算发酵液上清中蛋白质的残留量,即吸附后的蛋白损失率。筛选出能充分吸附大肠杆菌破碎液中蛋白质、多肽等大分子物质的吸附剂并优化吸附条件。结果表明吸附剂用量控制在6%~8%,pH值控制在4.0~4.5之间,对蛋白质的活性影响不大,蛋白备用液中蛋白质的回收率均能达到90%以上。

Research on E.coli Lysate Protein Adsorption Property of Natural Adsorbents

In this study,E.coli BL21(pQHP) was cultured and induced,and the fermentation broth was centrifuged to gain E.coli cell.And then cells were disrupted to be miscellaneous protein solution.Diatomite,activated floridin and bentonite were used as adsorbents to adsorb protein.The remained protein concentration was analyzed by Bradford assay to assess adsorption efficiency.The best adsorbents and adsorbing condition were screened.When adsorbent dosage was 6-8%,pH was 4.0-4.5,protein adsorption efficiency was more than 90% and protein activity had not been affected significantly.