| 原位杂交检测人工感染鸭体内鸭瘟病毒的复制 |
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| 引用本文： | 程安春,廖永洪,朱德康,汪铭书,袁桂萍,徐超,韩晓英.原位杂交检测人工感染鸭体内鸭瘟病毒的复制[J].病毒学报,2008,24(1):72-75. |
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| 作者姓名： | 程安春 廖永洪 朱德康 汪铭书 袁桂萍 徐超 韩晓英 |
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| 作者单位： | 四川农业大学,动物医学院,禽病防治研究中心,四川,雅安,625014;动物疫病与人类健康四川省重点实验室,四川,雅安,625014;四川农业大学,动物医学院,禽病防治研究中心,四川,雅安,625014 |
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| 基金项目： | 教育部跨世纪优秀人才培养计划，国家自然科学基金，四川省杰出青年学科带头人培养计划，四川省重点学科建设项目 |
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| 摘 要： | 鸭瘟(Duck plague,DP)是由鸭瘟病毒(Duck plague virus,DPV)引起的鸭、鹅和天鹅的一种急性败血性传染病,发病率和死亡率甚高,是养鸭业的一大危害1-2].
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| 关 键 词： | 原位杂交 鸭瘟强毒 实验感染鸭 复制 |
| 文章编号： | 1000-8721（2008）01-0072-04 |
| 收稿时间： | 2006-11-03 |
| 修稿时间： | 2007-10-08 |
Replication of Duck Plague Virus in Artificially Infected Ducks Detected by in situ Hybridization |
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| CHENG An-chun,LIAO Yong-hong,ZHU De-kang,WANG Ming-shu,YUAN Gui-ping,XU Chao,HAN Xiao-ying.Replication of Duck Plague Virus in Artificially Infected Ducks Detected by in situ Hybridization[J].Chinese Journal of Virology,2008,24(1):72-75. |
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| Authors: | CHENG An-chun LIAO Yong-hong ZHU De-kang WANG Ming-shu YUAN Gui-ping XU Chao HAN Xiao-ying |
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| Institution: | Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625014, China. chenganchun@vip.163.om |
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| Abstract: | Replication of duck plague virus(DPV) in artificially infected ducks were detected by in situ hybridization(ISH)which employed a 37bp oligonucleotide as probe designed according to DPV DNA sequence in GenBank.The results indicated that DPV DNA was detected in liver,intestine and bursa Fabricius at 4 h,in spleen and esophagus at 6h,in thymus at 12h post infection;DPV DNA in lung and kidney was detected only in dead ducks and no positive signal was detected in muscle,heart,cerebrum and pancreas.DPV DNA was distributed in cell nucleus and cytoplasm.Hepatocytes,sinus endodermal cells and Kuffer's cells were the mainly infected cell types in liver.DPV DNA was mainly detected in epithelium of villi,in lamina propria of intestinal villi of duodenum,in stratum spinosum of esophagus,and in epithelium,cortex,medulla of bursa Fabricius.The positive signals were mainly detected in medulla of thymus,lymphocytes and macrophages of spleen.The research suggests that ISH is a direct and specific method in detecting DPV DNA in paraffin sections and it's also a good method for virus diagnosis and DNA location of DPV. |
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| Keywords: | in situ hybridization virulent duck plague virus artificially infected replication in ducks |
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