Short term viability of soft tissue detached from the skeleton of reef-building corals |
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Authors: | Isabelle Domart-Coulon Sylvie Tambutté Eric Tambutté |
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Institution: | a Centre Scientifique de Monaco, av. Saint Martin, MC 98 000 Monaco b UMR 1112 UNSA-INRA Faculté des Sciences, Parc Valrose B.P.71, F-06108 Nice Cedex 2, France |
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Abstract: | We have induced soft tissue detachment from the skeleton of two colonial hard corals of the Pocilloporid family, both in vivo and in vitro. A parallel was made between polyp “bail-out”, i.e. field and laboratory-observed detachment of tissue fragments alone from the skeleton, and the dissociation method used for initiation of coral primary cell cultures. The in vitro approach provided insights into the active cellular re-arrangement mechanisms underlying coral tissue detachment. Functional polyps were not regenerated. Viability of tissue isolates detached from coral skeleton was probed for their use as a model for short-term biological assays. Cell viability dropped from 70% to 30% within the first week maintenance in vitro. Short-term isolate cultures limited to 3 days are a compromise allowing attachment of coral cells, yet preserving viability at about 70% of the total coral cell population. |
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Keywords: | Coral cell culture Pocilloporidae Scleractinia Polyp bail-out Viability |
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