Immunochemical detection and characterisation of osteocalcin from moa bone |
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| Authors: | N L Huq S M Rambaud L C Teh A D Davies B McCulloch M M Trotter G E Chapman |
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| Affiliation: | 1. National Hormone Laboratory, Department of Biochemistry, University of Auckland, Private Bag, Auckland, New Zealand;2. Canterbury Museum, Christchurch, New Zealand;1. National Institute for Laser, Plasma and Radiation Physics, Magurele, Ilfov, Romania;2. ELI-NP, “Horia Hulubei” National Institute for Physics and Nuclear Engineering, Magurele, Ilfov, Romania;3. Faculty of Biology, University of Bucharest, Bucharest, Romania;4. Research Institute of the University of Bucharest (ICUB), University of Bucharest, Bucharest, Romania;1. Kessler Foundation, West Orange, NJ;2. Department of Physical Medicine and Rehabilitation, Rutgers, New Jersey Medical School, Newark, NJ;3. Kessler Institute for Rehabilitation, West Orange, NJ;4. Robert Wood Johnson Medical School, Rutgers University, New Brunswick, NJ;1. Harvard University, USA;2. University of York, United Kingdom;1. Istanbul Protocol Project in the Basque Country (IPP-BC) Working Group, Granstuevej 13, DK-2840 Holte, Denmark;2. Section of Forensic Pathology, Basque Institute of Legal Medicine, University of the Basque Country, Spain |
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| Abstract: | Osteocalcin (the 6,000 dalton Mr gamma-carboxyglutamate-containing protein of bone) has been detected in acid extracts of bones of the extinct class of New Zealand ratite birds, the moas, using a radioimmunoassay for sheep osteocalcin. The immunoreactive osteocalcin of the extracts of two of these bones (the fibulae from two specimens of Pachyornis elephantopus found in South Island swamps) has been fractionated by gel filtration chromatography and reversed-phase high performance liquid chromatography, and behaves in a manner characteristic of osteocalcin from modern bones. Carbon-14 dating of bones and gizzard contents found in association with these specimens indicates approximate ages of 3,600 and 7,400 years respectively. |
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