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A new approach for achievement of inulin accumulation in suspension cultures of Jerusalem artichoke (Helianthus tuberosus) using biotic elicitors |
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| Authors: | HS Taha AM Abd El-Kawy M Abd El-Kareem Fathalla |
| Institution: | 1. Plant Biotechnology Department, National Research Centre, Dokki, Cairo, Egypt;2. Plant Department, Science Collage for Girls, Al-Azhr University, Cairo, Egypt;3. Plant Resources Department, City for Scientific Research and Technology Applications, Borg El-Arab, Alexandria, Egypt |
| Abstract: | A promising protocol for achievement the accumulation rate of inulin compound in a suspension culture of Jerusalem artichoke (Helianthus tuberosus) was established. The effect of incorporated of cell cultures in combining with two type of biotic elicitors Aspergillus niger extract and Methyl-Jasmonate incorporation feeding medium on leaf cell growth patterns and production of inulin was investigated. The maximum value of cell growth parameters and highest content of inulinase activity (0.395 u/ml) were resulted from elicitation of augmented MS-medium with A. niger extract at the level of 0.2% in combination with Methyl-Jasmonate (150 μM) as compared with other concentrations after 2 weeks of cultivation. The chemical analyses of the different cell lines were spectro-photometerically performed. This study clearly indicates that combining of A. niger and Methyl-Jasmonate elicitors plays a critical role on inulin process and its accumulation in Jerusalem artichoke cell cultures. |
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