Absorbed substrate fermentation for pectinase production with Aspergillus niger

Summary A 130 litre packed-bed bioreactor was used for pectinase production with Aspergillus niger using absorbed substrate fermentation techniques. Pectinolytic enzyme activity and relative CO₂ production were used as indicators of metabolic activity. Absorbed substrate fermentation is an efficient process for pectinase production and is also an interesting model because the culture medium, water, nutrients and specific inducers, can be designed at the desired concentrations.