High frequency plant regeneration from stem explants of Brassica alboglabra Bailey in vitro |
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| Authors: | E.-C. Pua T. H. Trinh N.-H. Chua |
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| Affiliation: | (1) Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021-6399 New York, New York, USA;(2) Present address: Institute of Molecular and Cell Biology, The National University of Singapore, 0511 Kent Ridge, Singapore;(3) Present address: Institut de physiologie végétale, CNRS, 91190 Gif sur Yvette, France |
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| Abstract: | Culture conditions for shoot regeneration and proliferation, and rooting of Brassica alboglabra Bailey were optimized by a judicious selection of explants and manipulation of hormonal combinations in the culture medium. Both half and whole stem explants were more regenerative than cotyledons and hypocotyls. The highest shoot regeneration frequency (100%) accompanied by high number of shoots was obtained using half stem explants grown on Murashige & Skoog [14] medium supplemented with 2 mgl-1 BA in combination with 1 mgl-1 NAA, or 4 mgl-1 2iP with 0.5 mgl-1 NAA. For shoot proliferation, 4 mgl-1 kinetin was most effective. The presence of auxin reduced shoot proliferation significantly. Maximum rooting (100%) of shoot cuttings was obtained either in the absence or in the presence of 0.5 mgl-1 NAA, or IBA or IAA ranging from 0.1 to 8 mgl-1. |
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| Keywords: | Brassica alboglabra Chinese kale growth regulators plant regeneration tissue culture |
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