Efficient regeneration from cotyledon protoplasts in Arabidopsis thaliana |
| |
Authors: | A. Dovzhenko C. Dal Bosco J. Meurer H. U. Koop |
| |
Affiliation: | (1) Bereich Botanik, Department Biologie I, Ludwig-Maximilians-Universität, Munich, DE |
| |
Abstract: | Summary. An efficient and fast regeneration system from cotyledon protoplasts was established for Arabidopsis thaliana accessions C24, Columbia, and Wassilewskija. Culture conditions and media compositions were optimised for the development of protoplasts embedded in thin alginate layers. Unexpectedly, the absence of cytokinins had a positive effect on cell development. Moreover, combined adjustment of -naphthylacetic acid and dicamba concentrations resulted in high plating efficiencies of up to 30%, followed by shoot regeneration within only 19 days after protoplast isolation. The protocol is reproducible, efficient, extremely fast, and regenerated plants are fertile. Thus, this cotyledon-based system could prove useful for studying plant cell and molecular biology in A. thaliana.Correspondence and reprints: Zentrum für angewandte Biowissenschaften, Sonnenstrasse 5, 79104 Freiburg, Federal Republic of Germany.Received December 9, 2002; accepted April 13, 2003; published online September 23, 2003 |
| |
Keywords: | :Arabidopsis thaliana Cotyledon Protoplast culture Shoot regeneration. |
本文献已被 PubMed SpringerLink 等数据库收录! |
|