ROSA26 mice carry a modifier of Min-induced mammary and intestinal tumor development |
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Authors: | Rebecca L Kohlhepp Laura F Hegge Jeniel E Nett Amy R Moser |
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Institution: | (1) Department of Human Oncology, K4/330 CSC 3684, 600 Highland Avenue, University of Wisconsin-Madison, Madison, Wisconsin 53792, USA, US |
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Abstract: | B6.129S7-Gtrosa26 (B6.R26) mice carry a LacZ-neoR insertion on Chromosome (Chr) 6, made by promoter trapping with 129 ES cells. Female C57BL/6J Apc
Min
/+ (B6Min/+) mice are highly susceptible to intestinal tumors and to the induction of mammary tumors after treatment with ethylnitrosourea
(ENU). However, B6.R26/+Min/+ females develop fewer mammary and intestinal tumors after ENU treatment than do B6 Min/+ mice. B6.R26/+ mice from two independently derived congenic lines show this modifier effect. Each of these congenic lines
carries approximately 20 cM of 129-derived DNA flanking the insertion, raising the possibility that the resistance is due
to a linked modifier locus. To further map the modifier locus, we have generated several lines of mice carrying different
regions of the congenic interval. We have found that resistance to mammary and intestinal tumors in ENU-treated Min/+ mice maps to a minimum 4-cM interval that includes the ROSA26 LacZ-neoR insertion. Therefore, the resistance to tumor development is due to either the ROSA26 insertion or a very tightly linked
modifier locus.
Received: 10 May 2000 / Accepted: 25 July 2000 |
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