Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC) |
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| Authors: | Abadi Reza Haji Hosseini Baghdad |
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| Affiliation: | Department of Biology, Faculty of Science, Payame Noor University, Nakhel St. Lashkarak Rd, Tehran, 19569, Iran. hosseini@pnu.ac.ir |
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| Abstract: | In this paper, analysis of free nucleotides from mouseliver tissue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO4(-), subjected to preliminary purification on a Cu(2+)-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolution of free nucleotides on a single column. |
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