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Two amidophosphoribosyltransferase genes of Arabidopsis thaliana expressed in different organs |
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| Authors: | Toshiro Ito Hideaki Shiraishi Kiyotaka Okada Yoshiro Shimura |
| Affiliation: | (1) Division 1 of Gene Expression and Regulation, National Institute for Basic Biology, Myodaijicho, 444 Okazaki, Japan;(2) Department of Biophysics, Faculty of Science, Kyoto University, 606 Kyoto, Japan;(3) Present address: Department of Chemistry, Faculty of Science, Kyoto University, 606 Kyoto, Japan |
| Abstract: | Amidophosphoribosyltransferase (ATase: EC 2.4.2.14) is a key enzyme in the pathway of purine nucleotide biosynthesis. We have identified several cDNA clones whose amino acid sequences exhibit similarity with the known ATases in a cDNA library of young floral buds of Arabidopsis thaliana. The cDNA clones are derived from two genes homologous with each other. These cDNAs represent the first plant representatives of ATase gene. Structural comparison with ATases of other organisms has revealed that the two genes encode [4Fe-4S] cluster-dependent ATases. Northern blot analysis showed that expression level of the genes is different in three organs; one gene is expressed in flowers and roots, while the other gene is mainly expressed in leaves. |
| Keywords: | amidophosphoribosyltransferase (ATase: EC 2.4.2.14) Arabidopsis thaliana cold plaque screening purine nucleotide biosynthesis |
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