Effect of hypophysectomy, adrenalectomy, pituitary hormone secretion and gastric acid secretion on neurotensin induced gastric protection against stress gastric lesions |
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| Authors: | D E Hernandez G A Mason J W Adcock R C Orlando A J Prange |
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| Affiliation: | 1. North Carolina State University, School of Veterinary Medicine, Raleigh, NC 27606, USA;2. Biological Sciences Research Center, USA;3. Dept. of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC 27514, USA;4. Psychiatry and University of North Carolina School of Medicine, Chapel Hill, NC 27514, USA;5. Neurobiology Program, University of North Carolina School of Medicine, Chapel Hill, NC 27514, USA;1. Advanced Innovation Center for Human Brain Protection, National Clinical Research Center for Geriatric Disorders, Xuanwu Hospital, Capital Medical University, Beijing, China;2. Aging Translational Medicine Center, Xuanwu Hospital, Capital Medical University, Beijing, China;3. Key Laboratory of RNA Science and Engineering, CAS Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences and China National Center for Bioinformation, Beijing, China;4. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China;5. University of Chinese Academy of Sciences, Beijing, China;6. Sino-Danish College, University of Chinese Academy of Sciences, Beijing, China;7. State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China;8. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China;1. Department of Inorganic Technology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 821 37 Bratislava, Slovakia;2. Department of Physical Chemistry, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Białystok, Poland;3. J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejškova 2155/3, 18223 Prague, Czech Republic;4. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 821 37 Bratislava, Slovakia;5. Department of Inorganic Materials, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 821 37 Bratislava, Slovakia;1. Department of Molecular and Translational Medicine, University of Brescia, Italy;2. Center for Neural Science, New York University, New York, United States;3. Molecular Markers Laboratory, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy;1. School of Medicine, Southeast University, No. 87 Dingjiaqiao road, 210009, Nanjing, Jiangsu Province, China;2. Sports Medicine Center, Department of Orthopaedic Surgery, Zhongda Hospital Affiliated to Southeast University, No. 87 Dingjiaqiao road, 210009, Nanjing, Jiangsu Province, China;1. Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran;2. Institute of Chemical Technologies and Analytics, Division of Structural Chemistry, TU Wien, Getreidemarkt 9/164-SC, A-1060 Vienna, Austria;3. Analytical Instrumentation Center, Technische Universität Wien, Getreidemarkt 9, 1060 Wien, Austria |
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| Abstract: | In previous studies we have established that intracisternal (i.c.) but not peripheral (intravenous) administration of neurotensin (NT), a brain and gastrointestinal tridecapeptide, totally prevents the development of gastric lesions produced by cold-restraint stress (CRS) with food-deprived rats. In this investigation, removal of the pituitary and adrenal gland, anterior pituitary hormone secretion and gastric acid secretion were evaluated independently as potential intermediates for NT's protective effect. NT (30 micrograms) produced a significant reduction of gastric lesions incidence and severity in intact and sham-operated controls. Adrenalectomy, but not hypophysectomy totally blocked the protective effect of i.c. NT. In addition, replacement therapy with s.c. prednisone (1 mg/kg) for 5 days following adrenalectomy did not restore the protective activity of central (i.c.) NT in adrenalectomized rats. A significant reduction of serum levels of TSH, PRL and GH following i.c. NT (30 micrograms) was observed after 2h of CRS. The gastrosecretory studies revealed that i.c. NT (30 micrograms) did not affect gastric acid secretion in pylorus ligated rats. However, blockade of peripheral (gut) cholinergic (muscarinic) receptors with i.p. atropine methylbromide (1 mg/kg) significantly raised gastric pH and reduced gastric acid concentration and output. In conclusion, the results of this study indicate that the acute protective effect of brain NT appears to be mediated, at least in part, by the sympathoadrenomedullary axis, and not by the pituitary gland or substances derived from the pituitary or by inhibition of gastric acid secretion. |
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