Plant transformation: a simple particle bombardment device based on flowing helium |
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| Authors: | Y. Takeuchi M. Dotson N. T. Keen |
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| Affiliation: | (1) Department of Plant Pathology, University of California, 92521 Riverside, CA, USA |
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| Abstract: | We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants. |
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| Keywords: | transformation particle gun soybean GUS gene |
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