Molecular modeling of the effects of mutant alleles on chalcone synthase protein structure |
| |
Authors: | Christopher D. Dana David R. Bevan Brenda S. J. Winkel |
| |
Affiliation: | (1) Department of Biology and Fralin Biotechnology Center, Virginia Tech, Blacksburg, VA 24061, USA;(2) Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA;(3) Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA |
| |
Abstract: | Chalcone synthase (CHS) catalyzes the first committed step in flavonoid biosynthesis, a major pathway of plant secondary metabolism. An allelic series for the Arabidopsis CHS locus, tt4, was previously characterized at the gene, protein, and end-product levels. In an effort to deduce the molecular basis for the observed phenotypes, homology models were generated for five of the tt4 proteins based on the crystal structure of CHS2 from Medicago. Molecular dynamics simulations provided insights into how even those substitutions that are not in close spatial proximity to key functional residues may still alter the architecture and dynamic movement of the enzyme, with dramatic effects on enzyme function. Simulations carried out at different temperatures pointed to optimized positioning of key residues in the active site or dimerization domain, rather than enhancement of overall structure, as underlying the higher activity of two temperature-sensitive variants at lower temperatures. Extending this type of analysis to account for protein–protein interactions may offer additional insights into the mechanisms by which single amino-acid substitutions can affect diverse aspects of protein function.Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users |
| |
Keywords: | Homology modeling Molecular dynamics simulation Allelic series Chalcone synthase transparent testa Arabidopsis thaliana |
本文献已被 PubMed SpringerLink 等数据库收录! |
|