O-Dealkylation of coumarin and resorufin ethers by unicellular green algae: kinetic properties of Chlorella fusca and Chlorella sorokiniana |
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Authors: | F. Thies L. H. Grimme |
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Affiliation: | (1) Institute of Cell Biology, Biochemistry, and Biotechnology, Department of Biology/Chemistry, University of Bremen, D-28334 Bremen, Germany |
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Abstract: | O-Dealkylations of resorufin and coumarin ethers, mediated by microsomal cytochrome P450 mono-oxygenases from animals, plants and microorganisms, are shown here to be performed also by intact cells of the unicellular green algaeChlorella fusca andChlorella sorokiniana. The activity of theO-dealkylation of these ethers was up to tenfold higher withChlorella sorokiniana. Both algae dealkylated methyl-, ethyl-, and pentylethers of resorufin and coumarin. Dealkylation in vivo indicated efficient absorption of methoxy- and ethoxyresorufin, confirmed by the respective absorption kinetics. Piperonylbutoxide and 1-aminobenzotriazole, known inhibitors of plant and mammalian cytochrome P450s, significantly inhibited theO-dealkylase activity of both algal strains. The use of synchronized cultures of both algae revealed that efficiency ofO-dealkylation depends on the stage of the cell cycle: during the growth phase, theO-dealkylase activities increased more than proportional, and the distinct drop in activity during the last hours of the light period indicated the appearance of an endogenous substrate. |
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Keywords: | Cytochrome P450 O-Dealkylation 7-Ethoxyresorufin-O-dealkylase 7-Ethoxycoumarin-O-dealkylase Alga Chlorella |
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