Interaction between Tubulin and Na2+,K2+-ATPase in Brain Stem Neurons |
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Authors: | N M Vladimirova E N Sautkina T V Ovchinnikova N A Potapenko |
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Institution: | (1) Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia |
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Abstract: | Functionally active Na2+,K2+-ATPase isozymes containing three types of the catalytic subunits ( 1, 2, and 3) were obtained from calf brain by two methods: selective removal of contaminating proteins according to Jorgensen (1974) and selective solubilization of the enzyme with subsequent reformation of the membrane structure according to Esmann (1988). All preparations were characterized with respect to ouabain-inhibition constants. The presence of the cytoskeleton protein tubulin ( 3 isoform) in the high-molecular-weight complex of Na2+,K2+-ATPase 3 1 isozyme from brain stem axolemma and the junction between Na2+,K2+-ATPase 3 subunit and tubulin 3 subunit are shown for the first time. |
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Keywords: | Na2+ K2+-ATPase isoforms isozymes axolemma tubulin |
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